The Mouse ApoA2 (Apolipoprotein A2) ELISA Kit is designed for the quantitative detection of Apolipoprotein A2 levels in various biological samples from mouse models. Apolipoprotein A2 is a significant component of high-density lipoprotein (HDL) in mice and plays a crucial role in lipid metabolism and transport, contributing to the regulation of cholesterol homeostasis and cardiovascular health.
Accurate measurement of Apolipoprotein A2 levels is essential for studying lipid-related disorders, atherosclerosis, and cardiovascular diseases in mouse models. Assay Genie's Mouse ApoA2 ELISA Kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under strict quality control standards, this kit delivers reliable performance and is user-friendly, providing researchers with a valuable tool to quantify Apolipoprotein A2 levels accurately in mouse samples. Trust Assay Genie's Mouse ApoA2 ELISA Kit for dependable measurements in your lipid metabolism and cardiovascular research studies.
Product Name:
Mouse ApoA2 (Apolipoprotein A2) ELISA Kit
SKU:
AEES00196
Target:
Mouse ApoA2 (Apolipoprotein A2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ApoA2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ApoA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ApoA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ApoA2. You can calculate the concentration of Mouse ApoA2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
94-107
90-101
Average (%)
93
99
96
1:4
Range (%)
87-99
87-98
85-95
Average (%)
92
94
90
1:8
Range (%)
86-98
97-111
85-96
Average (%)
91
105
90
1:16
Range (%)
100-111
91-104
88-98
Average (%)
105
98
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-108
101
EDTA plasma (n=5)
94-106
102
Cell culture media (n=5)
85-96
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.84
2.36
9.17
0.92
2.16
9.36
Standard deviation
0.05
0.11
0.48
0.05
0.11
0.48
C V (%)
5.95
4.66
5.23
5.43
5.09
5.13
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse ApoA2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ApoA2 in samples. No significant cross-reactivity or interference between Mouse ApoA2 and analogues was observed.
