The Mouse ApoA1 (Apolipoprotein A1) ELISA Kit is meticulously crafted to enable the precise and sensitive quantification of Apolipoprotein A1 levels in various mouse biological samples.
Apolipoprotein A1 is a major component of high-density lipoprotein (HDL) particles and plays a crucial role in lipid metabolism and cholesterol transport. As a fundamental factor in cardiovascular health, ApoA1 is involved in the reverse cholesterol transport pathway, contributing to the prevention of atherosclerosis and cardiovascular diseases. With exceptional sensitivity and specificity, our Mouse ApoA1 ELISA Kit from Assay Genie ensures accurate and reproducible results, essential for insightful investigations into lipid metabolism, cardiovascular health, and related disorders. Manufactured under stringent quality control measures, this kit delivers robust and reliable performance, offering researchers a user-friendly and efficient tool for exploring ApoA1-related mechanisms and potential therapeutic targets.
Product Name:
Mouse ApoA1 (Apolipoprotein A1) ELISA Kit
SKU:
AEES00211
Target:
Mouse ApoA1 (Apolipoprotein A1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ApoA1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ApoA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ApoA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ApoA1. You can calculate the concentration of Mouse ApoA1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-98
85-97
101-112
Average (%)
93
92
105
1:4
Range (%)
97-105
98-107
87-96
Average (%)
101
103
93
1:8
Range (%)
93-105
99-109
102-113
Average (%)
97
103
105
1:16
Range (%)
93-108
94-104
98-110
Average (%)
101
99
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-108
103
EDTA plasma (n=5)
83-97
91
Cell culture media (n=5)
101-111
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.92
4.49
19.7
1.93
4.28
16.05
Standard deviation
0.11
0.21
0.91
0.1
0.2
0.73
C V (%)
5.73
4.68
4.62
5.18
4.67
4.55
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse ApoA1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ApoA1 in samples. No significant cross-reactivity or interference between Mouse ApoA1 and analogues was observed.
