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Mouse Ankyrin Repeat Domain Protein 1 (ANKRD1) ELISA Kit

SKU:
MODL00077
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9CR42
Sensitivity:
0.052ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CARP,ALRP,C-193,CVARP,MCARP,Ankyrin Repeat Domain 1(Cardiac Muscle),Cardiac ankyrin repeat protein,Cytokine-inducible gene C-193,Cytokine-inducible nuclear protein
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

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Mouse Ankyrin Repeat Domain Protein 1 (ANKRD1) ELISA Kit

The Mouse ANKRD1 (Ankyrin Repeat Domain Protein 1) ELISA Kit is specifically designed for the accurate detection of ANKRD1 levels in mouse serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring precise and reproducible results for a variety of research applications.ANKRD1 is a key protein involved in various cellular processes, including gene expression regulation, cellular differentiation, and response to stress. Its dysregulation has been linked to a variety of diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.

This ELISA kit offers researchers a reliable tool to quantitatively measure ANKRD1 levels in mouse samples, providing valuable insights into its role in disease development and progression. With its ease of use and high performance, this kit is an essential addition to any laboratory conducting research on ANKRD1 and its associated pathways.

Product Name:Mouse Ankyrin Repeat Domain Protein 1 (ANKRD1) ELISA Kit
Product Code:MODL00077
Size:96 Assays
Target:ANKRD1
Synonyms:CARP, ALRP, C-193, CVARP, MCARP, Ankyrin Repeat Domain 1(Cardiac Muscle), Cardiac ankyrin repeat protein, Cytokine-inducible gene C-193, Cytokine-inducible nuclear protein
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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