The Mouse ANG2 (Angiopoietin-2) CLIA Kit is a comprehensive solution for the precise measurement of Angiopoietin-2 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable tool for various research applications.Angiopoietin-2 is a key protein involved in regulating angiogenesis and vascular development, influencing processes such as endothelial cell migration and vascular permeability. Its dysregulation is associated with various diseases, including cancer, cardiovascular disorders, and inflammatory conditions.
Therefore, the Mouse ANG2 CLIA Kit is essential for studying the role of Angiopoietin-2 in these diseases and exploring potential therapeutic interventions.Overall, the Mouse ANG2 CLIA Kit from Assay Genie provides researchers with a reliable and efficient method for quantifying Angiopoietin-2 levels in mouse samples, contributing to a better understanding of angiogenesis-related processes and disease mechanisms.
Product Name:
Mouse ANG2 (Angiopoietin 2) CLIA Kit
SKU:
MOES00075
Target:
Mouse ANG2 (Angiopoietin 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ANG2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ANG2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ANG2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ANG2. You can calculate the concentration of Mouse ANG2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-113
98-112
97-111
Average (%)
107
106
103
1:4
Range (%)
93-108
92-104
96-113
Average (%)
99
97
104
1:8
Range (%)
94-107
100-116
84-98
Average (%)
99
108
90
1:16
Range (%)
100-110
98-114
99-117
Average (%)
105
105
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
95
EDTA plasma (n=5)
86-99
93
Cell culture media (n=5)
99-114
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.54
107.18
332.15
46.76
112.14
339.35
Standard deviation
5.04
10.81
36.64
5.18
12.04
34.85
C V (%)
11.85
10.09
11.03
11.08
10.74
10.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ANG2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ANG2 in samples. No significant cross-reactivity or interference between Mouse ANG2 and analogues was observed.
