The Mouse ANG1 (Angiopoietin 1) ELISA Kit is specifically designed for the accurate quantification of Angiopoietin 1 levels in diverse mouse biological samples.
Angiopoietin 1 plays a vital role in regulating vascular development and stability by interacting with Tie2 receptor tyrosine kinase. This interaction is essential for angiogenesis, vascular maturation, and tissue repair, highlighting the significance of Angiopoietin 1 in various physiological processes. With Assay Genie's Mouse ANG1 ELISA Kit, researchers can expect precise and reliable results due to its exceptional sensitivity and specificity. This kit is manufactured under rigorous quality control standards to ensure robust performance, making it an ideal choice for investigating the role of Angiopoietin 1 in vascular development, angiogenesis, and related pathologies. The user-friendly nature of this kit further enhances its utility in research studies focused on vascular physiology and therapeutic interventions.
Product Name:
Mouse ANG1 (Angiopoietin 1) ELISA Kit
SKU:
AEES00209
Target:
Mouse ANG1 (Angiopoietin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ANG1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ANG1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ANG1. You can calculate the concentration of Mouse ANG1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
88-97
95-106
Average (%)
98
94
99
1:4
Range (%)
83-96
90-98
100-110
Average (%)
91
93
105
1:8
Range (%)
88-102
93-105
96-103
Average (%)
96
101
99
1:16
Range (%)
86-95
93-101
91-105
Average (%)
92
98
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-95
91
EDTA plasma (n=5)
84-100
92
Cell culture media (n=5)
87-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.5
5.84
22.61
2.49
5.7
22.66
Standard deviation
0.13
0.27
1.14
0.13
0.27
1.07
C V (%)
5.2
4.62
5.04
5.22
4.74
4.72
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse ANG1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ANG1 in samples. No significant cross-reactivity or interference between Mouse ANG1 and analogues was observed.
