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Mouse Amylase Alpha 2, Pancreatic (AMY2) ELISA Kit

SKU:
MODL00065
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P00688
Sensitivity:
4.1pg/mL
Range:
9.375-600pg/mL
ELISA Type:
Sandwich
Synonyms:
AMY2A,AMY2B,PA,1,4-alpha-D-glucan glucanohydrolase
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Amylase Alpha 2, Pancreatic (AMY2) ELISA Kit

The Mouse Amylase Alpha 2 (Pancreatic Amy2) ELISA Kit is a valuable tool for researchers looking to accurately measure levels of amylase alpha 2 in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and reproducible results for a variety of research applications.Amylase alpha 2 is an enzyme crucial for the digestion of carbohydrates, specifically breaking down starch into maltose. Its levels can provide important insights into pancreatic function and digestive health in mice.

By accurately measuring amylase alpha 2 levels, researchers can gain a better understanding of conditions related to pancreatic function and potentially develop new therapies.Overall, the Mouse Amylase Alpha 2 (Pancreatic Amy2) ELISA Kit is an essential tool for studying digestive health in mice and can be a valuable asset for advancing research in this area.

Product Name:Mouse Amylase Alpha 2, Pancreatic (AMY2) ELISA Kit
Product Code:MODL00065
Size:96 Assays
Target:AMY2
Synonyms:AMY2A, AMY2B, PA, 1,4-alpha-D-glucan glucanohydrolase
Detection Method:Sandwich
Range:9.375-600pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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