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Mouse Alpha-2-Heremans Schmid Glycoprotein (aHSG) ELISA Kit

SKU:
MODL00044
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P29699
Sensitivity:
3.64ng/mL
Range:
9.375-600ng/mL
ELISA Type:
Sandwich
Synonyms:
HSGa,A2HS,AHS,FETUA,HSG-A2,Fetuin-A,Alpha 2-HS Glycoprotein,Fetuin A,Alpha-2-Z-Globulin,Ba-alpha-2-Glycoprotein
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Alpha-2-Heremans Schmid Glycoprotein (aHSG) ELISA Kit

The Mouse Alpha-2-HS-Glycoprotein (AHSG) ELISA Kit is a reliable and accurate tool for detecting levels of AHSG in mouse serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures precise and reproducible results, making it an ideal choice for a variety of research applications.AHSG, also known as Fetuin-A, is a multifunctional glycoprotein involved in various physiological processes, including regulation of calcium homeostasis, inhibition of insulin receptor autophosphorylation, and modulation of inflammation.

Dysregulation of AHSG has been linked to metabolic disorders, cardiovascular diseases, and inflammatory conditions, highlighting its importance as a biomarker for studying these diseases and exploring potential therapeutic interventions.Overall, the Mouse AHSG ELISA Kit provides researchers with a valuable tool for investigating the role of AHSG in health and disease, offering reliable data for advancing our understanding of this important glycoprotein.

Product Name:Mouse Alpha-2-Heremans Schmid Glycoprotein (aHSG) ELISA Kit
Product Code:MODL00044
Size:96 Assays
Target:aHSG
Synonyms:HSGa, A2HS, AHS, FETUA, HSG-A2, Fetuin-A, Alpha 2-HS Glycoprotein, Fetuin A, Alpha-2-Z-Globulin, Ba-alpha-2-Glycoprotein
Detection Method:Sandwich
Range:9.37-600ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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