null

Mouse Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) ELISA Kit

SKU:
MODL00140
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q64726
Sensitivity:
0.48ng/mL
Range:
1.25-80ng/mL
ELISA Type:
Sandwich
Synonyms:
ZA2G,ZAG
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Mouse Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) ELISA Kit

The Mouse Alpha-2-Glycoprotein 1 (AZGP1) ELISA Kit is a reliable and easy-to-use assay designed for the precise detection of AZGP1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.AZGP1 is a zinc-binding protein that plays a crucial role in various physiological processes, including lipid metabolism, immune response, and cancer progression.

Its involvement in these processes makes it an important biomarker for studying metabolic disorders, immune system function, and cancer development.With its user-friendly protocol and efficient performance, the Mouse AZGP1 ELISA Kit is a valuable tool for researchers seeking to investigate the role of AZGP1 in biological processes and diseases, ultimately leading to potential therapeutic advancements.

Product Name:Mouse Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) ELISA Kit
Product Code:MODL00140
Size:96 Assays
Target:aZGP1
Synonyms:ZA2G, ZAG
Detection Method:Sandwich
Range:1.25-80ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products