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Mouse Aldehyde Dehydrogenase, Mitochondrial (ALDM) ELISA Kit

SKU:
MODL00054
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P47738
Sensitivity:
0.12ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
ALDH2,ALDH-E2,ALDHI,Aldehyde Dehydrogenase 2
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Aldehyde Dehydrogenase, Mitochondrial (ALDM) ELISA Kit

The Mouse Aldehyde Dehydrogenase Mitochondrial (ALDM) ELISA Kit is a powerful tool for the accurate quantification of ALDM levels in mouse tissue samples. This kit offers high sensitivity and specificity, delivering robust and consistent results for a variety of research applications.ALDM is an important enzyme involved in the metabolism of aldehydes, playing a vital role in detoxification processes within the mitochondria. Dysregulation of ALDM activity has been linked to various diseases, including liver disorders and cancer, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.

With its user-friendly protocol and reliable performance, the Mouse ALDM ELISA Kit is an essential tool for researchers investigating mitochondrial function and the role of ALDM in health and disease. Trust AssayGenie's expertise and cutting-edge technology to advance your research in this critical area.

Product Name:Mouse Aldehyde Dehydrogenase, Mitochondrial (ALDM) ELISA Kit
Product Code:MODL00054
Size:96 Assays
Target:ALDM
Synonyms:ALDH2, ALDH-E2, ALDHI, Aldehyde Dehydrogenase 2
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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