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Mouse Albumin (ALB) ELISA Kit

SKU:
MODL00049
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P07724
Sensitivity:
63ng/mL
Range:
156-10000ng/mL
ELISA Type:
Sandwich
Synonyms:
Serum albumin
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
€599
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Description

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Mouse Albumin (ALB) ELISA Kit

The Mouse Albumin (ALB) ELISA Kit is specifically designed for the precise measurement of albumin levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.Albumin is a vital protein that plays a crucial role in maintaining osmotic pressure and transporting important substances in the blood. Changes in albumin levels can indicate various medical conditions such as kidney disease, liver damage, and nutritional deficiencies.

Therefore, this ELISA kit serves as a valuable tool for studying these conditions and developing potential diagnostic and therapeutic strategies.Overall, the Mouse Albumin (ALB) ELISA Kit is a reliable and efficient solution for researchers looking to measure albumin levels in mouse samples with high precision and confidence.

Product Name:Mouse Albumin (ALB) ELISA Kit
Product Code:MODL00049
Size:96 Assays
Target:ALB
Synonyms:Serum albumin
Detection Method:Sandwich
Range:156-10000ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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