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Mouse aFGF PharmaGenie ELISA Kit (SBRS1274)

SKU:
SBRS1274
Product Type:
ELISA Kit
Reactivity:
Mouse
Applications:
ELISA
ELISA Type:
Sandwich
€749
Frequently bought together:

Description

system_update_altDatasheet
Product SKU:SBRS1274
Size:96T
Application:The antibody pair provided in this kit recognizes Mouse Fibroblast Growth Factor acidic (FGF1).
Uniprot:P61148
Gene ID:14164
Gene Names:FGF-1 / FGF1 / FGFA
Synonyms:Fibroblast growth factor 1 (FGF-1) (Acidic fibroblast growth factor) (aFGF) (Heparin-binding growth factor 1) (HBGF-1)
Target Species:Mouse
Compatible Sample Types:Cell Culture Supernatants, Plasma, Serum
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Range:12 pg/ml - 3000 pg/ml
Sensitivity:12 pg/ml
Recommended Dilution:2 fold
Storage/Stability:May be stored for up to 6 months at 2 to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
  1. Pre-Coated 96-well Strip Microplate
  2. Wash Buffer
  3. Stop Solution
  4. Assay Diluent(s)
  5. Lyophilized Standard
  6. Biotinylated Detection Antibody
  7. Streptavidin-Conjugated HRP
  8. TMB One-Step Substrate

Other materials and equipment required:

The Assay Genie Mouse aFGF PharmaGenie ELISA Kit (SBRS1274) will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ul volumes
  • Adjustable 1-25 ul pipettes for reagent preparation
  • 100 ul and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 ul of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 ul of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 ul of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul of Stop Solution to each well.
  11. Read at 450 nm immediately.

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