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Mouse Adrenergic Receptor Beta 3 (ADRb3) ELISA Kit

SKU:
MODL00035
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P25962
Sensitivity:
5.9pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
BETA3AR,B3AR,Beta-3 adrenoreceptor
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Mouse Adrenergic Receptor Beta 3 (ADRb3) ELISA Kit

The Mouse Adrenergic Receptor Beta 3 (ADRB3) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of ADRB3 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a wide range of research applications.ADRB3 is a critical receptor involved in the regulation of energy metabolism, lipolysis, and thermogenesis.

It plays a crucial role in conditions such as obesity, diabetes, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With its high performance and ease of use, the Mouse ADRB3 ELISA Kit is an essential tool for researchers aiming to investigate the role of ADRB3 in various physiological and pathological processes in mouse models.

Product Name:Mouse Adrenergic Receptor Beta 3 (ADRb3) ELISA Kit
Product Code:MODL00035
Size:96 Assays
Target:ADRb3
Synonyms:BETA3AR, B3AR, Beta-3 adrenoreceptor
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-9991
EDTA plasma (n=5)80-9989
Heparin plasma (n=5)82-9990
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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