Based on the information provided on the URL www.assaygenie.com/mouse-adenosine-kinase-adk-elisa-kit/, the Mouse Adenosine Kinase (ADK) ELISA Kit is specifically designed for the accurate detection of adenosine kinase levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.Adenosine kinase is a key enzyme involved in purine metabolism, playing a crucial role in regulating levels of adenosine, an important signaling molecule. Dysregulation of adenosine kinase has been implicated in various diseases, including cancer, neurological disorders, and inflammatory conditions.
As such, the Mouse ADK ELISA Kit serves as a valuable tool for studying the role of adenosine kinase in these diseases and potentially developing new therapeutic strategies.Overall, the Mouse Adenosine Kinase (ADK) ELISA Kit from AssayGenie provides researchers with a reliable and efficient means of analyzing adenosine kinase levels in mouse samples, facilitating advancements in the understanding and treatment of various health conditions.
Product Name:
Mouse Adenosine Kinase (ADK) ELISA Kit
Product Code:
MODL00028
Size:
96 Assays
Target:
ADK
Synonyms:
AK, Adenosine 5'-Phosphotransferase
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
