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Mouse Activating Transcription Factor 6 (ATF6) ELISA Kit

SKU:
MODL00125
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
F6VAN0
Sensitivity:
0.113ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
Processed cyclic AMP-dependent transcription factor ATF-6 alpha
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Activating Transcription Factor 6 (ATF6) ELISA Kit

The Mouse Activating Transcription Factor 6 (ATF6) ELISA Kit is a specialized assay designed for the precise measurement of ATF6 levels in mouse serum, plasma, and cell lysates. This kit offers exceptional sensitivity and accuracy, ensuring robust and consistent results for various research applications.ATF6 is a key transcription factor involved in the unfolded protein response pathway, regulating cellular stress responses and promoting cell survival.

Dysregulation of ATF6 has been linked to various diseases, including cancer, diabetes, and neurodegenerative disorders, making it a valuable biomarker for investigating these conditions and exploring therapeutic interventions.With its reliable performance and ease of use, the Mouse ATF6 ELISA Kit is an indispensable tool for researchers studying ATF6 signaling pathways and exploring its potential implications in disease pathogenesis.

Product Name:Mouse Activating Transcription Factor 6 (ATF6) ELISA Kit
Product Code:MODL00125
Size:96 Assays
Target:ATF6
Synonyms:Processed cyclic AMP-dependent transcription factor ATF-6 alpha
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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