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Mouse Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

SKU:
MODL00050
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q61490
Sensitivity:
0.13ng/mL
Range:
0.468-30ng/mL
ELISA Type:
Sandwich
Synonyms:
CD166,MEMD,Activated Leucocyte Cell Adhesion Molecule
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
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Description

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Mouse Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

The Mouse Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of ALCAM levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for researchers studying immune cell adhesion and migration processes.ALCAM is a cell adhesion molecule involved in immune responses and inflammation, playing a crucial role in leukocyte activation and migration.

Its dysregulation has been linked to various immune-related disorders and diseases, making it a valuable biomarker for studying these conditions and developing potential therapies.Overall, the Mouse Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit offers precise and reliable measurements of ALCAM levels, enabling researchers to gain valuable insights into immune cell function and potential therapeutic targets.

Product Name:Mouse Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit
Product Code:MODL00050
Size:96 Assays
Target:ALCAM
Synonyms:CD166, MEMD, Activated Leucocyte Cell Adhesion Molecule
Detection Method:Sandwich
Range:0.468-30ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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