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Mouse Acid Phosphatase 5, Tartrate Resistant (ACP5) ELISA Kit

SKU:
MODL00018
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q05117
Sensitivity:
28pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
TRAP,TrATPase,Tartrate Resistant Acid Phosphatase,Type 5 acid phosphatase
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Acid Phosphatase 5, Tartrate Resistant (ACP5) ELISA Kit

The Mouse Acid Phosphatase 5 Tartrate-Resistant (ACP5) ELISA Kit is a powerful tool for the quantitative detection of ACP5 levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.ACP5 is an important enzyme that plays a critical role in bone resorption and skeletal development. It is implicated in diseases such as osteoporosis, rheumatoid arthritis, and Paget's disease, making it a valuable biomarker for studying these conditions and exploring potential treatment options.

With easy-to-follow protocols and dependable performance, the Mouse ACP5 ELISA Kit is an essential tool for researchers investigating the roles of ACP5 in bone metabolism and related disorders. Trust Assay Genie for high-quality ELISA kits that deliver reliable results for your experimental needs. Visit our website to learn more about this innovative product.

Product Name:Mouse Acid Phosphatase 5, Tartrate Resistant (ACP5) ELISA Kit
Product Code:MODL00018
Size:96 Assays
Target:ACP5
Synonyms:TRAP, TrATPase, Tartrate Resistant Acid Phosphatase, Type 5 acid phosphatase
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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