Mouse ACE (Angiotensin I Converting Enzyme) CLIA Kit
The Mouse ACE (Angiotensin I-Converting Enzyme) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of ACE levels in mouse serum, plasma, and tissue samples. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.ACE is a key enzyme involved in the renin-angiotensin system, playing a crucial role in regulating blood pressure and cardiovascular function. Dysregulation of ACE activity has been implicated in various diseases such as hypertension, heart failure, and kidney disease, making it an important biomarker for studying these conditions and developing therapeutic interventions.
This Mouse ACE CLIA Kit offers researchers a powerful tool for studying the role of ACE in various physiological and pathological processes in mouse models, advancing our understanding of ACE-related diseases and potential treatment strategies.
Product Name:
Mouse ACE (Angiotensin I Converting Enzyme) CLIA Kit
SKU:
MOES00070
Target:
Mouse ACE (Angiotensin I Converting Enzyme)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ACE. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ACE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ACE, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ACE. You can calculate the concentration of Mouse ACE in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-116
84-98
101-117
Average (%)
107
91
108
1:4
Range (%)
96-110
88-103
97-110
Average (%)
102
94
102
1:8
Range (%)
85-96
96-108
91-104
Average (%)
91
102
97
1:16
Range (%)
97-112
87-101
94-111
Average (%)
105
93
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-112
106
EDTA plasma (n=5)
96-112
104
Cell culture media (n=5)
100-112
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
210.46
527.53
1713.7
220.93
524.69
1560.58
Standard deviation
18.42
51.54
119.62
27.42
50.58
147.01
C V (%)
8.75
9.77
6.98
12.41
9.64
9.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ACE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ACE in samples. No significant cross-reactivity or interference between Mouse ACE and analogues was observed.
