The Mouse ACE (Angiotensin I Converting Enzyme) ELISA Kit is a cutting-edge assay designed for the precise quantitative detection of ACE levels in various mouse biological samples. ACE plays a crucial role in the renin-angiotensin system, influencing blood pressure regulation, electrolyte balance, and cardiovascular health. Accurate measurement of ACE is essential for deepening our understanding of its involvement in cardiovascular physiology and pathophysiology related to hypertension, heart failure, and renal diseases. By employing Assay Genie's Mouse ACE ELISA Kit, researchers can expect outstanding sensitivity and specificity, ensuring accurate and reproducible results.
This kit is manufactured under stringent quality control standards to deliver reliable and robust performance, making it an invaluable tool for investigating the intricate mechanisms of ACE in cardiovascular health and exploring potential therapeutic strategies. Its user-friendly design enhances usability, making it an excellent choice for cardiovascular research studies.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ACE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ACE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ACE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ACE. You can calculate the concentration of Mouse ACE in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-106
89-97
100-107
Average (%)
103
94
103
1:4
Range (%)
85-94
92-103
100-111
Average (%)
91
96
105
1:8
Range (%)
97-112
97-112
96-109
Average (%)
104
105
104
1:16
Range (%)
92-103
86-95
96-109
Average (%)
97
91
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-100
97
EDTA plasma (n=5)
88-97
93
Cell culture media (n=5)
95-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
44.15
111.57
496.47
42.72
122.98
458.54
Standard deviation
2.21
5.4
21.15
2.23
6.08
19.53
C V (%)
5.01
4.84
4.26
5.22
4.94
4.26
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse ACE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ACE in samples. No significant cross-reactivity or interference between Mouse ACE and analogues was observed.
