The Mouse Aβ1-42 (Amyloid Beta 1-42) ELISA Kit is a cutting-edge tool designed for the precise quantification of amyloid beta 1-42 levels in various mouse-derived biological samples. Amyloid beta peptides, particularly Aβ1-42, are key players in the development and progression of neurodegenerative diseases, notably Alzheimer's disease, where Aβ1-42 is known for its implication in amyloid plaque formation and neuronal toxicity. With the Mouse Aβ1-42 ELISA Kit offered by Assay Genie, researchers gain an instrumental resource to delve into the intricate roles of amyloid beta 1-42 in neurodegenerative pathways.
This kit is characterized by exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under stringent quality control measures, the Mouse Aβ1-42 ELISA Kit provides robust performance, making it an excellent choice for cutting-edge research endeavors aimed at unraveling the mechanisms underpinning Alzheimer's disease and related neurodegenerative conditions.
Product Name:
Mouse Aβ1-42 (Amyloid Beta 1-42) ELISA Kit
SKU:
AEES00205
Target:
Mouse Aβ1-42 (Amyloid Beta 1-42)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Aβ1-42. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Aβ1-42 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Aβ1-42, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Aβ1-42. You can calculate the concentration of Mouse Aβ1-42 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
96-106
97-109
Average (%)
94
99
101
1:4
Range (%)
98-105
95-104
87-95
Average (%)
101
100
91
1:8
Range (%)
94-101
88-101
92-103
Average (%)
97
94
95
1:16
Range (%)
93-107
96-106
82-98
Average (%)
100
101
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-99
95
EDTA plasma (n=5)
85-94
90
Cell culture media (n=5)
97-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.1
21.66
98.11
8.24
21.52
85.62
Standard deviation
0.53
1.07
4.93
0.43
1.06
3.64
C V (%)
5.82
4.94
5.02
5.22
4.93
4.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse Aβ1-42 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Aβ1-42 in samples. No significant cross-reactivity or interference between Mouse Aβ1-42 and analogues was observed.
