The Mouse 15-LO (Arachidonate 15-Lipoxygenase) ELISA Kit is specifically designed for the accurate measurement of 15-LO levels in mouse serum, plasma, and cell culture supernatants. With a high sensitivity and specificity, this kit provides reliable and reproducible results, making it ideal for a variety of research applications.15-LO is an important enzyme involved in the metabolism of arachidonic acid, playing a key role in inflammatory processes and the production of lipid mediators.
Dysregulation of 15-LO has been linked to various diseases such as inflammation, cancer, and cardiovascular disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Mouse 15-LO (Arachidonate 15-Lipoxygenase) ELISA Kit is a valuable tool for researchers seeking to better understand the role of 15-LO in health and disease, providing accurate and precise measurements for insightful research findings.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse 15-LO. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse 15-LO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse 15-LO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse 15-LO. You can calculate the concentration of Mouse 15-LO in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
84-96
96-108
Average (%)
99
91
103
1:4
Range (%)
93-108
85-99
89-104
Average (%)
99
91
95
1:8
Range (%)
93-105
83-94
84-94
Average (%)
99
89
89
1:16
Range (%)
92-108
87-100
83-94
Average (%)
98
92
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
101
EDTA plasma (n=5)
94-110
101
Cell culture media (n=5)
90-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.38
261.45
957.96
97.94
249.79
976.99
Standard deviation
5.63
15.5
37.94
6.81
12.51
32.83
C V (%)
5.67
5.93
3.96
6.95
5.01
3.36
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse 15-LO concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse 15-LO in samples. No significant cross-reactivity or interference between Mouse 15-LO and analogues was observed.
