The Monomethyl Histone H4 R3 Polyclonal Antibody (CAB3158) is a powerful tool for researchers studying epigenetics and chromatin modifications. This rabbit-derived antibody is highly specific to monomethylated histone H4 at arginine 3 (H4R3me1) and is validated for use in a variety of applications, including Western blotting and immunohistochemistry.Histone modifications, such as methylation, play a crucial role in gene expression and regulation. The monomethylation of histone H4 at arginine 3 is associated with transcriptional activation and is involved in various cellular processes, including DNA repair and cell cycle progression.
The CAB3158 antibody allows for the detection and analysis of this specific histone modification, providing valuable insights into how epigenetic changes impact gene expression and cellular function.Researchers in the fields of epigenetics, cancer biology, and developmental biology will find the Monomethyl Histone H4 R3 Polyclonal Antibody (CAB3158) to be a valuable tool for their studies. Its high specificity and reliability make it an essential component in investigating the role of histone modifications in various biological processes.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
Purification Method:
Affinity purification
Gene ID:
8359
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MonoMethyl-Histone H4-R3 Rabbit pAb (CAB3158).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
