The Monomethyl-Histone H3 (R8) Polyclonal Antibody (CAB3156) is a vital tool for researchers studying epigenetics and chromatin modifications. This antibody, produced from rabbit hosts, specifically recognizes the monomethylation of histone H3 at arginine 8 and is validated for use in applications such as immunofluorescence and chromatin immunoprecipitation (ChIP).Histone modification at arginine 8 is known to regulate gene expression, impacting various cellular processes such as DNA repair, replication, and transcription. The specificity of CAB3156 for monomethyl-histone H3 (R8) enables precise detection and analysis of this key modification, allowing researchers to investigate its role in development, disease, and other biological pathways.
With its ability to target a specific histone modification, the Monomethyl-Histone H3 (R8) Polyclonal Antibody (CAB3156) is a valuable tool for studies in epigenetics, cancer research, and other fields where chromatin remodeling plays a critical role. Its high reactivity with human samples makes it a versatile option for researchers looking to explore the intricate mechanisms of gene regulation at the chromatin level.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MonoMethyl-Histone H3-R8 Rabbit pAb (CAB3156) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
