The Monomethyl Histone H3 (R26) Polyclonal Antibody (CAB3163) is a key tool for researchers studying epigenetics and chromatin remodeling. This antibody, generated in rabbits, specifically targets the monomethylated form of histone H3 at arginine 26, a critical post-translational modification involved in gene regulation and chromatin structure.Histone modifications, such as monomethylation of histone H3 at R26, play a crucial role in regulating gene expression and chromatin dynamics. By targeting this specific histone modification, researchers can investigate the role of epigenetic regulation in various biological processes, including development, differentiation, and disease.
The Monomethyl Histone H3 (R26) Polyclonal Antibody is validated for use in techniques such as Western blotting and immunohistochemistry, enabling accurate detection and analysis of monomethylated histone H3 at R26 in a variety of sample types. Its high reactivity and specificity make it a valuable tool for studies in epigenetics, cancer biology, and other areas of research where chromatin remodeling and gene regulation are of interest.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MonoMethyl-Histone H3-R26 Rabbit pAb (CAB3163).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
