The Monomethyl Histone H3 R17 Polyclonal Antibody (CAB3151) is a valuable tool for researchers studying histone modifications and epigenetic regulation. This antibody, raised in rabbits, specifically targets monomethylated histone H3 at arginine 17, a modification associated with gene activation and transcriptional regulation.Histone modifications play a crucial role in chromatin structure and gene expression, and aberrant histone methylation has been linked to various diseases, including cancer. The Monomethyl Histone H3 R17 antibody allows for the detection and analysis of this specific histone modification in various cell types, providing insight into its role in cellular processes.
Validated for use in Western blot applications, this antibody is highly reactive with human samples, making it an ideal choice for studies in molecular biology, cancer research, and epigenetics. By understanding the impact of histone methylation at specific sites like H3 R17, researchers can unravel the complex regulatory mechanisms underlying gene expression and potentially identify new therapeutic targets for disease intervention.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells, using MonoMethyl-Histone H3-R17 Rabbit pAb (CAB3151) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
