The Monomethyl Histone H3 (K9) Polyclonal Antibody (CAB2358) is a valuable tool for researchers studying epigenetics and chromatin modifications. This antibody, raised in rabbits, is highly specific for detecting monomethylated Histone H3 at lysine 9 (H3K9me1) in human, mouse, and rat samples. It has been validated for use in techniques such as Western blot and immunofluorescence, allowing for accurate and reliable detection of this specific histone modification.Histone H3 lysine 9 methylation is a key epigenetic mark involved in regulating gene expression, chromatin structure, and cellular differentiation. Dysregulation of H3K9 methylation has been implicated in various diseases, including cancer and neurological disorders.
By targeting the monomethylated form of Histone H3 at lysine 9, researchers can gain insight into the role of this histone modification in disease progression and potentially identify novel therapeutic targets.This antibody is an essential tool for researchers interested in studying the impact of histone modifications on gene expression and chromatin remodeling. Its high specificity and sensitivity make it suitable for a wide range of applications in epigenetics research, providing valuable insights into the complex mechanisms underlying gene regulation and disease development.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using MonoMethyl-Histone H3-K9 Rabbit pAb (CAB2358) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
