The Monomethyl Histone H3 (K4) Polyclonal Antibody (CAB2355) is a valuable tool for research involving histone modifications, particularly monomethylation of histone H3 at lysine 4. This antibody, raised in rabbits, is highly specific to monomethylated histone H3 at K4, allowing for precise detection and analysis in various experimental settings such as chromatin immunoprecipitation (ChIP) and immunofluorescence.Histone modifications play a crucial role in regulating gene expression and chromatin structure, with monomethylation of histone H3 at K4 being associated with transcriptional activation. By targeting this specific histone modification, researchers can investigate how gene regulation is influenced in processes such as development, differentiation, and disease.
The Monomethyl Histone H3 (K4) Polyclonal Antibody is ideal for studies in epigenetics, cancer research, and developmental biology. Its high reactivity with human samples, along with its validation for techniques like Western blot and immunohistochemistry, make it a versatile tool for exploring the complex interplay between histone modifications and cellular processes. This antibody is a valuable asset for researchers seeking to unravel the epigenetic mechanisms underlying various biological phenomena.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MonoMethyl-Histone H3-K4 Rabbit pAb (CAB2355) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
