The Monomethyl Histone H3 (K14) Polyclonal Antibody (CAB5277) is a valuable tool for researchers studying epigenetics and chromatin modifications. This antibody, produced in rabbits, specifically recognizes histone H3 monomethylated at lysine 14 (H3K14me1) in various sample types, making it ideal for use in immunofluorescence, immunoprecipitation, and chromatin immunoprecipitation (ChIP) assays.Histone H3 is a core histone protein that plays a crucial role in packaging DNA into chromatin, and post-translational modifications of histone H3, such as methylation at lysine 14, are known to regulate gene expression.
The presence of H3K14me1 has been linked to transcriptional activation and gene regulation, making this antibody a valuable tool for studying gene expression and epigenetic mechanisms.By targeting H3K14me1, researchers can gain insights into the role of histone modifications in various cellular processes, including development, differentiation, and disease. This antibody's high specificity and sensitivity make it a reliable tool for researchers interested in unraveling the complexities of epigenetic regulation and chromatin dynamics.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MonoMethyl-Histone H3-K14 Rabbit pAb (CAB5277) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
