The Monomethyl Histone H2B K5 Polyclonal Antibody (CAB17552) is a vital tool for researchers studying epigenetics and chromatin modifications. This antibody, produced in rabbits, possesses high specificity and sensitivity for detecting the monomethylated form of Histone H2B at lysine 5 in human samples.Histone modifications, such as monomethylation of H2B K5, play a crucial role in gene expression regulation and chromatin structure dynamics. The CAB17552 antibody enables precise detection and analysis of this specific histone modification, allowing researchers to investigate its impact on cellular processes and potential involvement in diseases like cancer and developmental disorders.
The Monomethyl Histone H2B K5 Polyclonal Antibody is validated for use in Western blot applications, providing reliable and reproducible results. Its versatility in detecting monomethylated Histone H2B K5 in different cell types makes it an invaluable tool for epigenetics and chromatin research. Gain deeper insights into the role of histone modifications in gene regulation with this highly specific antibody.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity.
Purification Method:
Affinity purification
Gene ID:
3017/8349
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells, using MonoMethyl-Histone H2B-K5 Rabbit pAb (CAB17552) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
