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Mono-Methyl-Histone H3 (K10) Antibody (PACO00060)

SKU:
PACO00060
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
WB
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
€269
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Description

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Mono-Methyl-Histone H3 (K10) Antibody (PACO00060)

The Mono-Methyl Histone H3 K10 Antibody (PACO00060) is a valuable tool for research in epigenetics and chromatin regulation. This polyclonal antibody, raised in rabbits, specifically targets the mono-methylated form of histone H3 lysine 10 (H3K10me1) and is highly reactive with human samples. Validated for use in techniques like Western blotting, this antibody allows for the detection and analysis of H3K10me1 levels in various cell types.Histone modifications, such as methylation of histone H3 at lysine 10, play a crucial role in regulating gene expression and chromatin structure. The mono-methylation of H3K10 is associated with transcriptional activation and has been implicated in various biological processes, including cell proliferation, differentiation, and cancer development.

Understanding the role of H3K10me1 in epigenetic regulation is essential for unraveling the complexity of gene expression and identifying potential therapeutic targets for diseases like cancer.Overall, the Mono-Methyl Histone H3 K10 Antibody (PACO00060) provides researchers with a powerful tool for investigating the role of histone modifications in gene regulation and chromatin dynamics, offering insights into epigenetic mechanisms underlying various biological processes and diseases.

Antibody Name:Mono-Methyl-Histone H3 (K10) Antibody
Antibody SKU:PACO00060
Size:50ug
Host Species:Rabbit
Tested Applications:ELISA, WB
Recommended Dilutions:WB:1:500-1:2000
Species Reactivity:Human, Mouse, Rat
Immunogen:synthesized peptide derived from human Histone H3 around the mono-methylation site of K10.
Form:Liquid
Storage Buffer:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Synonyms:HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1;
UniProt Protein Function:H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 6p22.2

Cellular Component: extracellular region; membrane; nuclear chromosome; nuclear chromosome, telomeric region; nucleoplasm; nucleosome; nucleus; protein complex

Molecular Function:cadherin binding; histone binding; protein binding

Biological Process: blood coagulation; cellular protein metabolic process; chromatin silencing at rDNA; DNA replication-dependent nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; nucleosome assembly; positive regulation of gene expression, epigenetic; protein heterotetramerization; RNA-mediated gene silencing; telomere organization and biogenesis

NCBI Summary:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
UniProt Code:P68431
NCBI GenInfo Identifier:55977055
NCBI Gene ID:8357
NCBI Accession:P68431.2
UniProt Secondary Accession:P68431,P02295, P02296, P16106, Q6ISV8, Q6NWP8, Q6NWP9 Q6NXU4, Q71DJ3, Q93081, A0PJT7, A5PLR1,
UniProt Related Accession:P68431
Molecular Weight:15kDa
NCBI Full Name:Histone H3.1
NCBI Synonym Full Names:histone cluster 1 H3 family member h
NCBI Official Symbol:HIST1H3H  
NCBI Official Synonym Symbols:H3/k; H3FK; H3F1K  
NCBI Protein Information:histone H3.1
UniProt Protein Name:Histone H3.1
UniProt Synonym Protein Names:Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l
UniProt Gene Name:HIST1H3A  

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