The Mono-Methyl Histone H1 Lys25 Antibody (PACO00144) is a high-quality polyclonal antibody designed for research involving histone modification, specifically mono-methylation of lysine 25 on the histone H1 protein. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.Histone modifications, such as mono-methylation, play a critical role in regulating gene expression and chromatin structure. The mono-methylation of lysine 25 on histone H1 is known to impact chromatin compaction and gene regulation, making it an important target for studying epigenetic mechanisms involved in various cellular processes.
The Mono-Methyl Histone H1 Lys25 Antibody enables the detection and analysis of mono-methylated histone H1 lysine 25 in various cell types, providing researchers with a valuable tool for investigating the role of this histone modification in different biological contexts. This antibody is particularly well-suited for studies in epigenetics, gene regulation, and chromatin remodeling, offering insights into the complex interplay between histone modifications and cellular function.
Antibody Name:
Mono-Methyl-Histone H1 (Lys25) Antibody
Antibody SKU:
PACO00144
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
WB:1:500-1:1000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
synthetic Peptide
Form:
Liquid
Storage Buffer:
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
H1F1: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. Belongs to the histone H1/H5 family.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 6p21.3Cellular Component: nuclear chromatin; nucleosomeMolecular Function: chromatin DNA binding; protein bindingBiological Process: nucleosome assembly; spermatogenesis
UniProt Protein Details:
NCBI Summary:
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]
