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Mono-methyl-HIST1H2BC (K20) Antibody (PACO59662)

SKU:
PACO59662
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
WB
ChIP
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
  • Mono-methyl-HIST1H2BC K20 Antibody PACO59662
  • Mono-methyl-HIST1H2BC K20 Antibody PACO59662
€349
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Description

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Mono-methyl-HIST1H2BC (K20) Antibody (PACO59662)

The Mono-Methyl Hist1H2Bc (K20) Antibody (PAC05962) is a highly specific antibody designed for research involving histone modification, specifically the mono-methylation of lysine 20 on the Hist1H2Bc protein. This antibody, produced through immunization of rabbits, exhibits high reactivity with human samples and is validated for use in various applications such as Western blot and immunohistochemistry.Histone modifications, such as mono-methylation of lysine 20 on Hist1H2Bc, play a crucial role in regulating gene expression and chromatin structure, impacting various cellular processes including DNA repair, transcription, and cell cycle regulation.

The detection and analysis of this specific modification using the Mono-Methyl Hist1H2Bc (K20) Antibody is essential for studying epigenetic regulation and its implications in development, disease, and cellular function.Researchers investigating the role of histone modifications in cancer, gene regulation, and chromatin dynamics will find this antibody invaluable for their studies. Its specificity and reliability make it a valuable tool for understanding the complex mechanisms of epigenetic regulation and its impact on cellular processes.

Antibody Name:Mono-methyl-HIST1H2BC (K20) Antibody (PACO59662)
Antibody SKU:PACO59662
Size:50ul
Host Species:Rabbit
Tested Applications:ELISA, WB, ChIP
Recommended Dilutions:ELISA:1:2000-1:10000, WB:1:100-1:1000
Species Reactivity:Human
Immunogen:Peptide sequence around site of Mono-methyl-Lys (20) derived from Human Histone H2B type 1-C/E/F/G/I
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:Antigen Affinity Purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageWestern Blot. Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, A549 whole cell lysate. All lanes: HIST1H2BC antibody at 1:100. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 14 kDa. Observed band size: 14 kDa.
Product ImageChromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2BC (PACO59662) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta -Globin promoter.
Background:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Synonyms:Histone H2B type 1-C/E/F/G/I (Histone H2B.1 A) (Histone H2B.a) (H2B/a) (Histone H2B.g) (H2B/g) (Histone H2B.h) (H2B/h) (Histone H2B.k) (H2B/k) (Histone H2B.l) (H2B/l), HIST1H2BC; HIST1H2BE; HIST1H2BF; HIST1H2BG; HIST1H2BI, H2BFL; H2BFH; H2BFG; H2BFA; H2BFK
UniProt Protein Function:H2B1C: a core component of the nucleoosome. The nucleosome, a basic organizational unit of chromosomal DNA, is octrameric, consisting of two molecules each of histones H2B, H2A, H3, H4. The octamer wraps approximately 147 bp of DNA. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 6p22.1

Cellular Component: cytoplasm; extracellular space; nucleoplasm; nucleus

Molecular Function:DNA binding; protein binding

Biological Process: antibacterial humoral response; defense response to Gram-positive bacterium; innate immune response in mucosa; nucleosome assembly

NCBI Summary:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein has antibacterial and antifungal antimicrobial activity. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
UniProt Code:P62807
NCBI GenInfo Identifier:290457686
NCBI Gene ID:8339
NCBI Accession:P62807.4
UniProt Secondary Accession:P62807,P02278, Q3B872, Q4VB69, Q93078, Q93080,
UniProt Related Accession:P62807
Molecular Weight:13,906 Da
NCBI Full Name:Histone H2B type 1-C/E/F/G/I
NCBI Synonym Full Names:histone cluster 1, H2bg
NCBI Official Symbol:HIST1H2BG  
NCBI Official Synonym Symbols:H2B/a; H2BFA; H2B.1A; dJ221C16.8  
NCBI Protein Information:histone H2B type 1-C/E/F/G/I
UniProt Protein Name:Histone H2B type 1-C/E/F/G/I
UniProt Synonym Protein Names:Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; H2B/l
Protein Family:Histone
UniProt Gene Name:HIST1H2BC  
UniProt Entry Name:H2B1C_HUMAN
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