null

Mono-methyl-HIST1H2BC (K15) Antibody (PACO60497)

SKU:
PACO60497
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
WB
ICC
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
  • Mono-methyl-HIST1H2BC K15 Antibody PACO60497
  • Mono-methyl-HIST1H2BC K15 Antibody PACO60497
€349
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

Mono-methyl-HIST1H2BC (K15) Antibody (PACO60497)

The Mono-Methyl Hist1H2B-K15 Antibody (PAC060497) is a highly specific tool for researchers studying post-translational modifications of histone proteins. This antibody, generated in rabbit, is suitable for use in various experimental applications such as Western blot and immunofluorescence, providing reliable detection and analysis of mono-methylated Hist1H2B at lysine 15.Histone modifications, including mono-methylation, play a crucial role in regulating gene expression and chromatin structure, impacting various cellular processes such as DNA repair, replication, and transcription. The Mono-Methyl Hist1H2B-K15 Antibody allows researchers to investigate the role of this specific modification in epigenetic regulation and its implications in diseases such as cancer and developmental disorders.

With its high specificity and sensitivity, the Mono-Methyl Hist1H2B-K15 Antibody is a valuable tool for scientists conducting research in epigenetics, chromatin biology, and cancer biology. By facilitating the detection and analysis of mono-methylated Hist1H2B-K15, this antibody enables a deeper understanding of the molecular mechanisms underlying gene regulation and disease development.

Antibody Name:Mono-methyl-HIST1H2BC (K15) Antibody (PACO60497)
Antibody SKU:PACO60497
Size:50ul
Host Species:Rabbit
Tested Applications:ELISA, WB, ICC
Recommended Dilutions:ELISA:1:2000-1:10000, WB:1:100-1:1000, ICC:1:10-1:100
Species Reactivity:Human
Immunogen:Peptide sequence around site of Mono-methyl-Lys (15) derived from Human Histone H2B type 1-C/E/F/G/I
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:Antigen Affinity Purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageWestern Blot. Positive WB detected in: A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate. All lanes: HIST1H2BC antibody at 1:100. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 14 kDa. Observed band size: 14 kDa.
Product ImageImmunocytochemistry analysis of PACO60497 diluted at 1:10 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Synonyms:Histone H2B type 1-C/E/F/G/I (Histone H2B.1 A) (Histone H2B.a) (H2B/a) (Histone H2B.g) (H2B/g) (Histone H2B.h) (H2B/h) (Histone H2B.k) (H2B/k) (Histone H2B.l) (H2B/l), HIST1H2BC; HIST1H2BE; HIST1H2BF; HIST1H2BG; HIST1H2BI, H2BFL; H2BFH; H2BFG; H2BFA; H2BFK
UniProt Protein Function:H2B1C: a core component of the nucleoosome. The nucleosome, a basic organizational unit of chromosomal DNA, is octrameric, consisting of two molecules each of histones H2B, H2A, H3, H4. The octamer wraps approximately 147 bp of DNA. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 6p22.1

Cellular Component: cytoplasm; extracellular space; nucleoplasm; nucleus

Molecular Function:DNA binding; protein binding

Biological Process: antibacterial humoral response; defense response to Gram-positive bacterium; innate immune response in mucosa; nucleosome assembly

NCBI Summary:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein has antibacterial and antifungal antimicrobial activity. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
UniProt Code:P62807
NCBI GenInfo Identifier:290457686
NCBI Gene ID:8339
NCBI Accession:P62807.4
UniProt Secondary Accession:P62807,P02278, Q3B872, Q4VB69, Q93078, Q93080,
UniProt Related Accession:P62807
Molecular Weight:13,906 Da
NCBI Full Name:Histone H2B type 1-C/E/F/G/I
NCBI Synonym Full Names:histone cluster 1, H2bg
NCBI Official Symbol:HIST1H2BG  
NCBI Official Synonym Symbols:H2B/a; H2BFA; H2B.1A; dJ221C16.8  
NCBI Protein Information:histone H2B type 1-C/E/F/G/I
UniProt Protein Name:Histone H2B type 1-C/E/F/G/I
UniProt Synonym Protein Names:Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; H2B/l
Protein Family:Histone
UniProt Gene Name:HIST1H2BC  
UniProt Entry Name:H2B1C_HUMAN
Antibodies
Anti-Ubiquityl-Histone H2B-K120 Antibody (CAB17949)
Anti-HIST1H2BG Antibody (CAB9896)
Secondary Antibody
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)
Recommended Products
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011)
Anti-HRP-conjugated Beta Actin Antibody (CABC028)

Related Products