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Monkey Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit

SKU:
MKDL00037
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q58T13
Sensitivity:
0.36ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
CD106,INCAM-100,L1CAM
Reactivity:
Monkey
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Monkey Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit

The Monkey Vascular Cell Adhesion Molecule-1 (VCAM1) ELISA Kit is specifically designed for the accurate measurement of VCAM1 levels in monkey serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.VCAM1 is a key molecule involved in cell adhesion and plays a crucial role in inflammation and immune responses. Elevated levels of VCAM1 have been associated with various diseases, including cardiovascular disorders, autoimmune conditions, and cancer.

Therefore, this ELISA kit is essential for studying the role of VCAM1 in disease pathogenesis and for developing potential therapeutic interventions.Overall, the Monkey VCAM1 ELISA Kit is a valuable tool for researchers looking to investigate the function of VCAM1 and its involvement in various physiological and pathological processes in monkey models.

Product Name:Monkey Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit
Product Code:MKDL00037
Size:96 Assays
Target:VCAM1
Synonyms:CD106, INCAM-100, L1CAM
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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