The Monkey UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) ELISA Kit is a specialized assay designed for the precise and quantitative measurement of UCHL1 levels in monkey biological samples.
UCHL1 is a crucial enzyme involved in regulating protein degradation and maintaining cellular function, particularly in neuronal and immune cells. This ELISA kit enables researchers to accurately quantify UCHL1 concentrations in monkey samples, providing valuable insights into cellular protein homeostasis, neurodegeneration, and the immune response in primates. By assessing UCHL1 levels, researchers can gain a deeper understanding of neurodegenerative diseases, immune system function, and cellular stress responses in monkey models. With exceptional sensitivity and specificity, the Monkey UCHL1 ELISA Kit delivers reliable and reproducible results, allowing researchers to explore the role of UCHL1 in primate health and disease. Manufactured under rigorous quality control standards, this kit offers robust performance and user-friendly protocols, making it an excellent choice for cutting-edge research in primate neurobiology and immunology studies.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey UCHL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey UCHL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey UCHL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey UCHL1. You can calculate the concentration of Monkey UCHL1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
84-98
97-109
Average (%)
104
90
104
1:4
Range (%)
93-105
79-91
97-113
Average (%)
100
86
104
1:8
Range (%)
96-112
81-93
96-112
Average (%)
103
87
102
1:16
Range (%)
98-114
81-92
97-113
Average (%)
106
86
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
97
EDTA plasma (n=5)
94-110
101
Cell culture media (n=5)
84-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
254.36
433.05
2419.2
240.52
444.16
2508.75
Standard deviation
13.23
19.62
122.65
12.75
22.74
116.41
C V (%)
5.2
4.53
5.07
5.3
5.12
4.64
Sample type &Sample volume:
; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey UCHL1 concentrations in .
Specificity:
This kit recognizes Monkey UCHL1 in samples. No significant cross-reactivity or interference between Monkey UCHL1 and analogues was observed.
