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Monkey Ubiquitin (Ub) ELISA Kit

SKU:
MKDL00035
Product Type:
ELISA Kit
Sensitivity:
2.1ng/mL
Range:
6.25-400ng/mL
ELISA Type:
Sandwich
Synonyms:
Ubiquitin B,UbB,Ubiquitous Immunopoietic Polypeptide,Polyubiquitin B
Reactivity:
Monkey
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Monkey Ubiquitin (Ub) ELISA Kit

The Monkey Ubiquitin (UB) ELISA Kit is a reliable and accurate tool for detecting levels of ubiquitin in monkey serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.Ubiquitin is a key protein involved in regulating various cellular processes, including protein degradation, DNA repair, and immune response.

Dysregulation of ubiquitin has been linked to various diseases, such as cancer, neurodegenerative disorders, and inflammatory conditions, making it a valuable biomarker for studying these diseases and developing potential treatments.Overall, the Monkey Ubiquitin (UB) ELISA Kit is a valuable tool for researchers looking to study the role of ubiquitin in disease pathology and therapeutic development in monkeys.

Product Name:Monkey Ubiquitin (Ub) ELISA Kit
Product Code:MKDL00035
Size:96 Assays
Target:Ub
Synonyms:Ubiquitin B, UbB, Ubiquitous Immunopoietic Polypeptide, Polyubiquitin B
Detection Method:Sandwich
Range:6.25-400ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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