Monkey TRACP-5b (Tartrate Resistant Acid Phosphatase 5b) ELISA Kit (AEES00711)

Product Name:	Monkey TRACP-5b (Tartrate Resistant Acid Phosphatase 5b) ELISA Kit
SKU:	AEES00711
Target:	Monkey TRACP-5b (Tartrate Resistant Acid Phosphatase 5b)
Size:	96T
Assay type:	Sandwich-ELISA
Assay time:	3.5h
Sensitivity:	0.47 ng/mL
Detection range:	0.78-50 ng/mL

Kit component:

Component	Quantity (24 Assays)	Quantity (96 Assays)	Storage
Micro ELISA Plate (Dismountable)	8 wells x 3 strips	8 wells x 12 strips	-20°C, 12 months
Reference Standard	1 vial	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 60 µL	1 vial, 120 µL
Concentrated HRP Conjugate (100×)	1 vial, 60 µL	1 vial, 120 µL	-20°C (shading light), 12 months
Reference Standard & Sample Diluent	1 vial, 20 mL	1 vial, 20 mL	4°C, 12 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	1 vial, 10 mL	4°C (shading light)
Stop Solution	1 vial, 10 mL	1 vial, 10 mL	4°C
Plate Sealer	5 pieces	5 pieces
Product Description	1 copy	1 copy

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey TRACP-5b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey TRACP-5b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey TRACP-5b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey TRACP-5b. You can calculate the concentration of Monkey TRACP-5b in the samples by comparing the OD of the samples to the standard curve.

Linearity:

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	97-109	95-111	91-106
1:2	Average (%)	104	102	97
1:4	Range (%)	99-113	97-111	91-106
1:4	Average (%)	105	105	98
1:8	Range (%)	96-111	90-107	88-98
1:8	Average (%)	102	98	93
1:16	Range (%)	94-108	94-109	94-110
1:16	Average (%)	100	100	100

Recovery:

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	87-99	94
EDTA plasma (n=5)	89-104	95
Cell culture media (n=5)	96-112	102

Precision:

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20.0	20.0	20.0	20.0	20.0
Mean (ng/mL)	2.36	4.82	22.71	2.18	5.21	20.84
Standard deviation	0.13	0.22	1.09	0.12	0.28	1.04
C V (%)	5.51	4.56	4.8	5.5	5.37	4.99

Sample type &Sample volume:	; 100μL
Reproducibility:	Both intra-CV and inter-CV are < 10%.
Application:	This ELISA kit applies to the in vitro quantitative determination of Monkey TRACP-5b concentrations in .
Specificity:	This kit recognizes Monkey TRACP-5b in samples. No significant cross-reactivity or interference between Monkey TRACP-5b and analogues was observed.