The Monkey TRACP-5b (Tartrate Resistant Acid Phosphatase 5b) ELISA Kit is a specialized assay designed for the precise quantitative measurement of TRACP-5b levels in monkey biological samples. TRACP-5b is an important enzyme involved in bone metabolism and is known for its role in osteoclast activity and bone resorption processes.
This ELISA kit provides researchers with a valuable tool to assess TRACP-5b levels in monkey samples, facilitating investigations into bone turnover, osteoclast differentiation, and bone resorption activities. TRACP-5b is a reliable marker for osteoclast function and bone resorption, making it a key target for studies related to bone health and bone-related disorders in primates. With exceptional sensitivity and specificity, the Monkey TRACP-5b ELISA Kit ensures accurate and reproducible results, enabling researchers to gain valuable insights into bone metabolism and osteoclast function in monkeys. Manufactured under stringent quality control standards, this kit offers robust performance and ease of use, making it an excellent choice for research studies focused on bone health and skeletal biology in primate models.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey TRACP-5b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey TRACP-5b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey TRACP-5b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey TRACP-5b. You can calculate the concentration of Monkey TRACP-5b in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
95-111
91-106
Average (%)
104
102
97
1:4
Range (%)
99-113
97-111
91-106
Average (%)
105
105
98
1:8
Range (%)
96-111
90-107
88-98
Average (%)
102
98
93
1:16
Range (%)
94-108
94-109
94-110
Average (%)
100
100
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
94
EDTA plasma (n=5)
89-104
95
Cell culture media (n=5)
96-112
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.36
4.82
22.71
2.18
5.21
20.84
Standard deviation
0.13
0.22
1.09
0.12
0.28
1.04
C V (%)
5.51
4.56
4.8
5.5
5.37
4.99
Sample type &Sample volume:
; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey TRACP-5b concentrations in .
Specificity:
This kit recognizes Monkey TRACP-5b in samples. No significant cross-reactivity or interference between Monkey TRACP-5b and analogues was observed.
