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Monkey Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit

SKU:
MKDL00033
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q5PXZ9
Sensitivity:
0.108ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
SFD,TIMP Metallopeptidase Inhibitor 3,Metallopeptidase Inhibitor 3,Sorsby Fundus Dystrophy,Pseudoinflammatory
Reactivity:
Monkey
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€749
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Description

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Monkey Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit

The Monkey Tissue Inhibitors of Metalloproteinase-3 (TIMP3) ELISA Kit is a reliable tool for detecting TIMP3 levels in monkey tissue samples. This kit offers high sensitivity and specificity, providing accurate and reproducible results for a variety of research applications.TIMP3 is a key inhibitor of metalloproteinases, enzymes that break down extracellular matrix proteins. Dysregulation of TIMP3 has been linked to various pathologies, including cancer, inflammation, and cardiovascular diseases.

By measuring TIMP3 levels in monkey tissues, researchers can gain valuable insights into the role of TIMP3 in disease processes and potential therapeutic interventions. Whether studying angiogenesis, cell proliferation, or extracellular matrix remodeling, the Monkey TIMP3 ELISA Kit is an essential tool for researchers looking to advance their understanding of TIMP3 biology in monkey models.

Product Name:Monkey Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit
Product Code:MKDL00033
Size:96 Assays
Target:TIMP3
Synonyms:SFD, TIMP Metallopeptidase Inhibitor 3, Metallopeptidase Inhibitor 3, Sorsby Fundus Dystrophy, Pseudoinflammatory
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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