The Monkey PTH (Parathyroid Hormone) ELISA Kit is specifically designed for the precise measurement of parathyroid hormone levels in monkey serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, allowing for consistent and accurate results, making it ideal for various research purposes.Parathyroid hormone is a vital hormone that regulates calcium and phosphate levels in the blood, influencing bone metabolism and kidney function. Imbalances in parathyroid hormone levels can lead to conditions such as hyperparathyroidism or hypoparathyroidism, highlighting the importance of monitoring its levels in research settings.
The Monkey PTH ELISA Kit is a valuable tool for studying the role of parathyroid hormone in bone health, mineral metabolism, and related disorders in monkeys. With its reliable performance and ease of use, researchers can rely on this kit to advance their research in the field of endocrinology and bone health.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey I-PTH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey I-PTH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey I-PTH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey I-PTH. You can calculate the concentration of Monkey I-PTH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-97
96-111
98-111
Average (%)
90
102
105
1:4
Range (%)
93-106
83-96
87-98
Average (%)
98
89
93
1:8
Range (%)
91-107
86-99
85-97
Average (%)
98
93
92
1:16
Range (%)
87-98
83-94
84-98
Average (%)
92
89
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
95
EDTA plasma (n=5)
93-108
101
Cell culture media (n=5)
86-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.98
120.33
420.51
51.73
121.3
461.88
Standard deviation
2.99
5.29
16.4
2.99
5.74
20.0
C V (%)
5.44
4.4
3.9
5.78
4.73
4.33
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey I-PTH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey I-PTH in samples. No significant cross-reactivity or interference between Monkey I-PTH and analogues was observed.
