The Monkey PA (Prealbumin) ELISA Kit is specifically designed for the precise measurement of prealbumin levels in monkey samples, including serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, providing researches with accurate and reproducible results for various applications.Prealbumin, also known as transthyretin, is an important protein involved in transporting thyroxine and vitamin A in the blood.
Changes in prealbumin levels can serve as indicators for malnutrition, liver disease, and inflammation, making it a valuable biomarker for monitoring health and disease progression in monkeys.With the Monkey PA ELISA Kit, researchers can effectively study the role of prealbumin in various physiological and pathological conditions, ultimately leading to a better understanding of monkey health and potential therapeutic interventions.
Product Name:
Monkey PA (Prealbumin) ELISA Kit (MKES00038)
SKU:
MKES00038
Target:
Monkey PA (Prealbumin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 μg/mL
Detection range:
0.31-20 μg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey TTR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey TTR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey TTR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey TTR. You can calculate the concentration of Monkey TTR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
90-106
99-112
Average (%)
104
97
104
1:4
Range (%)
99-112
92-109
96-111
Average (%)
105
100
102
1:8
Range (%)
94-108
90-105
94-108
Average (%)
102
96
102
1:16
Range (%)
99-114
89-104
97-114
Average (%)
105
95
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
100
EDTA plasma (n=5)
89-105
96
Cell culture media (n=5)
87-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.99
1.71
8.2
0.89
1.78
7.78
Standard deviation
0.06
0.07
0.26
0.05
0.09
0.34
C V (%)
6.06
4.09
3.17
5.62
5.06
4.36
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey TTR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey TTR in samples. No significant cross-reactivity or interference between Monkey TTR and analogues was observed.
