Monkey NTXI (Cross Linked N-telopeptide of Type I Collagen) ELISA Kit (MKES00044)
The Monkey NTxI Cross-Linked N Telopeptide of Type I Collagen ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of NTxI levels in monkey samples. This kit offers reliable and reproducible results, making it ideal for a wide range of research applications.NTxI is a key marker of bone resorption and turnover, providing valuable insights into bone health and metabolism.
Monitoring NTxI levels can help researchers better understand bone-related conditions such as osteoporosis, osteopenia, and bone metastases, and evaluate the effectiveness of potential treatments.Overall, the Monkey NTxI Cross-Linked N Telopeptide of Type I Collagen ELISA Kit is a valuable tool for studying bone metabolism and assessing bone health in experimental monkey models.
Product Name:
Monkey NTXI (Cross Linked N-telopeptide of Type I Collagen) ELISA Kit (MKES00044)
SKU:
MKES00044
Target:
Monkey NTXI (Cross Linked N-telopeptide of Type I Collagen)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey NTXI. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey NTXI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey NTXI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey NTXI. You can calculate the concentration of Monkey NTXI in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
88-102
88-101
Average (%)
101
94
94
1:4
Range (%)
91-104
88-103
90-101
Average (%)
98
95
95
1:8
Range (%)
90-101
90-101
82-94
Average (%)
96
96
89
1:16
Range (%)
97-113
88-102
88-102
Average (%)
103
93
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
96
EDTA plasma (n=5)
94-108
100
Cell culture media (n=5)
94-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.19
5.0
0.51
1.27
5.31
Standard deviation
0.04
0.06
0.18
0.03
0.06
0.18
C V (%)
7.55
5.04
3.6
5.88
4.72
3.38
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey NTXI concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey NTXI in samples. No significant cross-reactivity or interference between Monkey NTXI and analogues was observed.
