The Monkey LDL (Low Density Lipoprotein) ELISA Kit is a specialized assay designed for the precise quantitative measurement of Low Density Lipoprotein levels in monkey biological samples.
LDL is a crucial class of lipoproteins that transport cholesterol in the bloodstream and are integral to lipid metabolism and the development of atherosclerosis. This ELISA kit provides researchers with a reliable tool to study the levels of LDL in monkey samples, offering insights into cholesterol metabolism, cardiovascular health, and potential implications for cardiovascular disease research in primates. LDL is known to be a key player in lipid transport and plays a significant role in the development of cardiovascular conditions. With exceptional sensitivity and specificity, the Monkey LDL ELISA Kit ensures accurate and reproducible results, facilitating investigations into the role of LDL in primate health and disease. Manufactured under stringent quality control standards, this kit offers robust performance and ease of use, making it an excellent choice for research studies focusing on lipid metabolism and cardiovascular health in monkeys.
Product Name:
Monkey LDL (Low Density Lipoprotein) ELISA Kit
SKU:
AEES00710
Target:
Monkey LDL (Low Density Lipoprotein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 ng/mL
Detection range:
7.81-500 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey LDL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey LDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey LDL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey LDL. You can calculate the concentration of Monkey LDL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
96-111
96-111
Average (%)
104
101
103
1:4
Range (%)
97-110
92-106
94-110
Average (%)
103
99
102
1:8
Range (%)
96-111
93-106
96-110
Average (%)
103
98
103
1:16
Range (%)
92-109
97-111
97-113
Average (%)
100
104
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
97
EDTA plasma (n=5)
91-107
99
Cell culture media (n=5)
94-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.22
51.29
232.84
21.78
46.53
212.79
Standard deviation
1.22
2.13
11.8
1.28
2.72
10.96
C V (%)
5.25
4.15
5.07
5.88
5.85
5.15
Sample type &Sample volume:
; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey LDL concentrations in .
Specificity:
This kit recognizes Monkey LDL in samples. No significant cross-reactivity or interference between Monkey LDL and analogues was observed.
