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Monkey Interferon Beta (IFNb) ELISA Kit

SKU:
MKDL00016
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
B6CK14
Sensitivity:
7.1pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Competitive
Synonyms:
IFNB1,IFN-B,IFB,IFF,IFNB,Interferon Beta 1 Fibroblast
Reactivity:
Monkey
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Monkey Interferon Beta (IFNb) ELISA Kit

The Monkey IFN-Beta (IFNB) ELISA Kit is specifically designed for the precise measurement of interferon beta levels in monkey serum, plasma, and cell culture supernatants. This kit offers high sensitivity and accuracy, guaranteeing consistent and dependable results for various research applications.Interferon beta is a key cytokine involved in the immune response to viral infections and plays a crucial role in regulating inflammation.

Monitoring interferon beta levels can provide valuable insights into the immune system's response to pathogens and disease progression.With its reliable performance and ease of use, the Monkey IFN-Beta (IFNB) ELISA Kit is an essential tool for researchers studying immune responses, viral infections, and inflammatory diseases in monkey models.

Product Name:Monkey Interferon Beta (IFNb) ELISA Kit
Product Code:MKDL00016
Size:96 Assays
Target:IFNb
Synonyms:IFNB1, IFN-B, IFB, IFF, IFNB, Interferon Beta 1 Fibroblast
Detection Method:Competition
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.Aspirate and wash 3 times
4.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.Aspirate and wash 5 times
6.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.Add 50µL Stop Solution. Read at 450 nm immediately.

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