Monkey IgA (Immumoglobulin A) ELISA Kit (MKES00007)
The Monkey IgA (Immunoglobulin A) ELISA Kit is specially designed for the precise measurement of IgA levels in monkey serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, guaranteeing dependable and consistent results for various research purposes.Immunoglobulin A is a key component of the immune system, providing protection against infections by neutralizing pathogens and toxins.
Abnormal levels of IgA have been linked to autoimmune diseases, allergies, and various infections, making it a valuable biomarker for investigating these conditions and developing therapeutic interventions.Overall, the Monkey IgA ELISA Kit is a valuable tool for researchers studying immune responses and infectious diseases in monkey models, offering reliable and accurate data to advance scientific discoveries in this field.
Product Name:
Monkey IgA (Immumoglobulin A) ELISA Kit (MKES00007)
SKU:
MKES00007
Target:
Monkey IgA (Immumoglobulin A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey IgA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey IgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey IgA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey IgA. You can calculate the concentration of Monkey IgA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-112
88-99
86-96
Average (%)
105
94
91
1:4
Range (%)
88-98
83-96
83-94
Average (%)
93
88
89
1:8
Range (%)
89-100
86-96
89-100
Average (%)
94
91
94
1:16
Range (%)
88-102
83-95
86-98
Average (%)
94
89
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
96
EDTA plasma (n=5)
86-98
93
Cell culture media (n=5)
93-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.53
147.42
339.91
53.01
155.53
342.69
Standard deviation
2.97
7.83
11.42
3.03
7.85
18.54
C V (%)
6
5.31
3.36
5.72
5.05
5.41
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey IgA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey IgA in samples. No significant cross-reactivity or interference between Monkey IgA and analogues was observed.
