The Monkey IFN-Beta (Interferon-Beta) ELISA Kit is specifically designed for the quantitative detection of IFN-Beta in monkey serum, plasma, and cell culture supernatants. This ELISA kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.IFN-Beta is a type I interferon that plays a key role in the immune response against viral infections and autoimmune diseases. It is also involved in regulating inflammation and immune cell activation.
Detection of IFN-Beta levels can provide valuable insights into the immune response in monkeys, aiding in the study of infectious diseases, autoimmune disorders, and immunotherapy research.Overall, the Monkey IFN-Beta ELISA Kit is a valuable tool for researchers looking to study the role of IFN-Beta in monkey models, providing reliable data for advancing knowledge in immunology and developing potential therapies.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey IFN-β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey IFN-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey IFN-β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey IFN-β. You can calculate the concentration of Monkey IFN-β in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
91-105
93-105
Average (%)
99
99
98
1:4
Range (%)
88-99
83-95
87-99
Average (%)
93
88
93
1:8
Range (%)
88-99
83-97
90-101
Average (%)
93
90
95
1:16
Range (%)
89-103
81-95
84-95
Average (%)
96
88
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
99
EDTA plasma (n=5)
92-104
99
Cell culture media (n=5)
86-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.99
147.16
435.45
52.3
161.74
412.89
Standard deviation
3.34
8.34
14.28
3.31
7.76
18.83
C V (%)
6.96
5.67
3.28
6.33
4.8
4.56
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey IFN-β concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey IFN-β in samples. No significant cross-reactivity or interference between Monkey IFN-β and analogues was observed.
