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Monkey Galactosidase Beta (GLb) ELISA Kit

SKU:
MKDL00015
Product Type:
ELISA Kit
Sensitivity:
1.54U/L
Range:
3.125-200U/L
ELISA Type:
Sandwich
Synonyms:
GL-B,b-GAL,bGAL,ELNR1,EBP,GLB1,Lactase,Elastin Receptor 1,67kDa,Elastin Receptor 1(67kD),Acid Beta-Galactosidase
Reactivity:
Monkey
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Monkey Galactosidase Beta (GLb) ELISA Kit

The Monkey Galactosidase Beta (GLB) ELISA Kit is specifically designed for the quantitative detection of GLB levels in monkey serum, plasma, and cell culture supernatants. This kit offers high precision and accuracy, providing researchers with reliable and reproducible results for a variety of applications.Galactosidase Beta is an important enzyme involved in various biological processes, including carbohydrate metabolism and lysosomal storage disorders. Understanding the levels of GLB in biological samples can provide valuable insights into these processes and aid in the development of novel therapies.

Whether studying lysosomal storage disorders, carbohydrate metabolism, or other related research areas, the Monkey GLB ELISA Kit is a valuable tool for scientists looking to accurately measure GLB levels in monkey samples. Trust in the sensitivity and specificity of this kit to support your research endeavors.

Product Name:Monkey Galactosidase Beta (GLb) ELISA Kit
Product Code:MKDL00015
Size:96 Assays
Target:GLb
Synonyms:GL-B, b-GAL, bGAL, ELNR1, EBP, GLB1, Lactase, Elastin Receptor 1,67kDa, Elastin Receptor 1(67kD), Acid Beta-Galactosidase
Detection Method:Sandwich
Range:3.125-200U/L
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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