The Monkey F8 (Coagulation Factor VIII) ELISA Kit is a specialized assay designed to quantitatively detect and measure F8 levels in monkey biological samples. Coagulation Factor VIII plays a crucial role in the blood coagulation cascade, specifically in the intrinsic pathway, and deficiencies or abnormalities in F8 can lead to bleeding disorders and coagulopathies. This ELISA kit enables researchers to accurately quantify F8 levels in monkey samples, providing valuable insights into the coagulation process. Monitoring F8 levels is essential for assessing coagulation disorders, understanding hemostasis, and investigating conditions such as hemophilia A.
With exceptional sensitivity and specificity, the Monkey F8 ELISA Kit ensures precise and reproducible results, allowing researchers to study the coagulation pathway and its associated disorders in primate models. Manufactured under stringent quality control standards, this kit offers reliable performance and user-friendly protocols, making it an excellent choice for research focused on hemostasis and coagulation in monkeys.
Product Name:
Monkey F8 (Coagulation Factor Ⅷ) ELISA Kit
SKU:
AEES00713
Target:
Monkey F8 (Coagulation Factor Ⅷ)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey F8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey F8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey F8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey F8. You can calculate the concentration of Monkey F8 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
99-115
89-101
Average (%)
100
105
94
1:4
Range (%)
92-106
101-117
89-105
Average (%)
98
108
97
1:8
Range (%)
91-106
97-109
83-97
Average (%)
98
104
89
1:16
Range (%)
92-102
96-113
84-95
Average (%)
97
103
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-95
90
EDTA plasma (n=5)
93-109
99
Cell culture media (n=5)
87-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.96
2.62
8.97
0.93
2.66
8.32
Standard deviation
0.06
0.12
0.41
0.06
0.16
0.38
C V (%)
6.25
4.58
4.57
6.45
6.02
4.57
Sample type &Sample volume:
; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey F8 concentrations in .
Specificity:
This kit recognizes Monkey F8 in samples. No significant cross-reactivity or interference between Monkey F8 and analogues was observed.
