The Monkey F12 Coagulation Factor 12 ELISA Kit is specifically designed for the precise measurement of coagulation factor 12 levels in monkey serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it suitable for a variety of research applications.Coagulation factor 12 is a key player in the coagulation cascade, essential for proper blood clotting and hemostasis. Dysregulation of factor 12 levels can lead to bleeding disorders or thrombotic events, highlighting the importance of monitoring its levels in various disease states.
This ELISA kit provides researchers with a valuable tool for studying the role of coagulation factor 12 in thrombotic disorders, bleeding disorders, and other related conditions in monkey models. By accurately quantifying factor 12 levels, this kit can aid in understanding the underlying mechanisms of these diseases and potentially contribute to the development of new therapeutic interventions.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey F12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey F12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey F12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey F12. You can calculate the concentration of Monkey F12 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
95-109
94-106
Average (%)
97
100
101
1:4
Range (%)
91-106
95-110
89-104
Average (%)
97
102
96
1:8
Range (%)
86-101
92-105
92-107
Average (%)
93
97
98
1:16
Range (%)
88-98
94-107
95-111
Average (%)
93
101
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
91
EDTA plasma (n=5)
91-106
98
Cell culture media (n=5)
92-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.15
9.33
48.83
5.0
8.95
50.82
Standard deviation
0.36
0.39
1.58
0.25
0.42
1.77
C V (%)
6.99
4.18
3.24
5.0
4.69
3.49
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey F12 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey F12 in samples. No significant cross-reactivity or interference between Monkey F12 and analogues was observed.
