The Monkey F11 (Coagulation Factor XI) ELISA Kit is a powerful tool for detecting levels of Coagulation Factor XI in monkey serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and dependable results for various research applications.Coagulation Factor XI is a key protein involved in the blood clotting process, playing a crucial role in maintaining hemostasis and preventing excessive bleeding. Dysregulation of Factor XI has been linked to various bleeding disorders and thrombotic events, highlighting its importance as a biomarker for studying these conditions and developing targeted therapies.
The Monkey F11 (Coagulation Factor XI) ELISA Kit is a valuable resource for researchers looking to explore the role of Factor XI in coagulation disorders, cardiovascular diseases, and other related conditions in monkey models. Its reliable performance and ease of use make it an excellent choice for labs conducting preclinical studies and translational research.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey F11. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey F11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey F11, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey F11. You can calculate the concentration of Monkey F11 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-114
87-101
94-107
Average (%)
104
94
100
1:4
Range (%)
89-103
81-92
82-96
Average (%)
94
88
88
1:8
Range (%)
90-103
82-92
85-101
Average (%)
97
88
92
1:16
Range (%)
87-100
82-96
89-99
Average (%)
94
88
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-97
92
EDTA plasma (n=5)
89-102
96
Cell culture media (n=5)
87-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.47
6.66
16.97
2.49
6.83
17.14
Standard deviation
0.17
0.37
0.88
0.14
0.3
0.6
C V (%)
6.88
5.56
5.19
5.62
4.39
3.5
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey F11 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey F11 in samples. No significant cross-reactivity or interference between Monkey F11 and analogues was observed.
