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Monkey Cytochrome P450 1A1 (CYP1A1) ELISA Kit

SKU:
MKDL00007
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q6GUR1
Sensitivity:
0.056ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CYP1,AHH,AHRR,CP11,CYP1,P1-450,P450-C,P450DX,Cytochrome P450,Subfamily I(Aromatic Compound-Inducible),Polypeptide 1
Reactivity:
Monkey
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Monkey Cytochrome P450 1A1 (CYP1A1) ELISA Kit

The Monkey Cytochrome P450 1A1 (CYP1A1) ELISA Kit is a powerful tool for detecting levels of CYP1A1 in monkey samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and dependable results for a variety of research applications.Cytochrome P450 1A1 is an important enzyme involved in drug metabolism and detoxification processes in the body. Its expression is influenced by environmental factors such as exposure to toxins and pollutants, making it a valuable biomarker for studying the impact of these substances on health.

With the Monkey Cytochrome P450 1A1 (CYP1A1) ELISA Kit, researchers can easily quantify CYP1A1 levels in monkey samples, leading to a better understanding of its role in drug metabolism and potentially uncovering new therapeutic targets for various conditions.

Product Name:Monkey Cytochrome P450 1A1 (CYP1A1) ELISA Kit
Product Code:MKDL00007
Size:96 Assays
Target:CYP1A1
Synonyms:CYP1, AHH, AHRR, CP11, CYP1, P1-450, P450-C, P450DX, Cytochrome P450,Subfamily I(Aromatic Compound-Inducible),Polypeptide 1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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