Monkey CTXI (Cross Linked C-telopeptide of Type I Collagen) ELISA Kit (MKES00069)
The Monkey CTXI (Cross-linked C-telopeptide of Type I Collagen) ELISA Kit is specifically designed for the precise measurement of CTXI levels in monkey serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and accuracy, ensuring consistent and dependable results that are suitable for a variety of research purposes.CTXI is an important biomarker for assessing bone turnover and bone resorption processes. Elevated CTXI levels have been linked to conditions such as osteoporosis, bone fractures, and other bone-related disorders.
By measuring CTXI levels, researchers can gain valuable insights into bone health and potential treatment options.Overall, the Monkey CTXI ELISA Kit is a valuable tool for studying bone metabolism, identifying bone disorders, and developing therapeutic interventions. Its reliability, precision, and ease of use make it an essential asset for researchers in the field of bone biology and related disciplines.
Product Name:
Monkey CTXI (Cross Linked C-telopeptide of Type I Collagen) ELISA Kit (MKES00069)
SKU:
MKES00069
Target:
Monkey CTXI (Cross Linked C-telopeptide of Type I Collagen)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey CTXâ… . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey CTXâ… and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey CTXâ… , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey CTXâ… . You can calculate the concentration of Monkey CTXâ… in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
90-104
95-113
Average (%)
93
95
103
1:4
Range (%)
88-101
88-104
94-110
Average (%)
95
95
102
1:8
Range (%)
85-96
90-106
97-113
Average (%)
91
97
104
1:16
Range (%)
85-97
85-99
91-107
Average (%)
91
91
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
100
EDTA plasma (n=5)
90-104
95
Cell culture media (n=5)
85-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
1.35
3.97
0.52
1.45
4.1
Standard deviation
0.03
0.05
0.19
0.03
0.08
0.22
C V (%)
6.12
3.7
4.79
5.77
5.52
5.44
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey CTXâ… concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey CTXâ… in samples. No significant cross-reactivity or interference between Monkey CTXâ… and analogues was observed.
